The Batman legend takes an unexpected turn when the Caped Crusader vanishes from the crime-ridden city of New Gotham and his legacy is taken over by a trio of beautiful and relentless heroines - the Birds of Prey. Barbara Gordon started out as Batman's protege, Batgirl, but an attack by The Joker left her confined to a wheelchair. Reinventing herself as Oracle, she takes under her wing Helena Kyle, the secret daughter of Batman and Catwoman, who quickly grows into the fierce and beautiful "Huntress", and Dinah, a teenage runaway who is drawn to the city by meta-human visions. With the help of the only honest cop in New Gotham, Detective Jesse Reese, the Birds of Prey fight their first battle against a mysterious madwoman who is bent on their destruction.

Slick, an evil meta-human who can turn from solid to liquid, is hired by Dr. Quinzel to kill the honest cops who stand in her way. When Reese is the next intended victim, Helena comes to his aid and their mutual attraction continues to grow. Meanwhile, Barbara believes Dinah's story that she's just a runaway, but Helena remains suspicious.

Helena and Detective Reese clash again when they both try to track down a serial killer who destroys metahumans by taking on their powers. Meanwhile, Dinah ditches school when she learns from Helena there is a metahuman-only bar, and Barbara meets Wade's parents who think she is not normal enough for their son.

When Helena rescues an abandoned baby boy and brings him back to the Clocktower, Barbara and Dinah try to help, but to everyone's surprise, the baby, Guy, is only happy when Helena is holding him. Even more surprising is the fact that Guy wakes up from his nap as a walking, talking 5-year-old and is soon a teenager. Now in a race against time, the Birds of Prey discover that Guy is programmed to live his entire life in three days, and to kill the first person he attaches to, putting Helena in grave danger.

Barbara and Helena learn the truth about Dinah and Dinah learns the truth about her own past when her mother, Carolyn Lance, comes to New Gotham in search of the daughter she abandoned years before. Dinah is shocked to learn that Carolyn was once Batman's protege, Black Canary. Meanwhile, Helena learns that Detective Reese has family issues of his own when he becomes secretive about a ruthless gangster.

At the request of Det. Reese, Helena goes undercover to help bring down a gang of thieves terrorizing New Gotham and quickly discovers that the gang is part of a larger infrastructure. Helena goes after the big boss until her cover is blown, putting her in grave danger. Meanwhile, Barbara has to deal with her fear of intimacy when her relationship with Wade takes a serious turn.

Already frustrated with her difficult relationship with Reese, Helena is intrigued when she meets Darkstrike, a metahuman who has come to New Gotham on the trail of a serial killer. Meanwhile, Barbara and Dinah discover that Darkstrike has a dangerous alter ego.

Helena is thrilled when her best friend from high school, Sandra, returns to New Gotham, but the Birds are troubled by a series of murders with the unmistakable mark of Batgirl's nemesis, Lady Shiva. Determined to fight Lady Shiva as an equal, Barbara dusts off her Batgirl costume and experiments with technology that allows her to leave the wheelchair.

When a hit is put out on the life of legendary mob boss, Al Hawke, the only person he trusts to protect him is his son, Detective Reese. Reese turns to Helena for help, but when she learns that Reese's father is the same man who killed Dinah's mother, Black Canary, her loyalties are tested.

When young metahuman women are abducted in New Gotham, Helena's investigation leads her to uncover a secret club where the captive women are drugged and forced to fight each other in an arena. When Helena is also abducted, it is up to Dinah to rescue her.

When Helena's former classmates descend upon New Gotham for their fifth class reunion, two of the alums are brutally murdered. While Helena tries to find the killer, she fears that Reese's investigation will lead him to learn too much about her true identity.

After a robber, Chris Cassius, breaks into a chemical factory and drinks a mixture that gives him the power to turn people to stone, Barbara cancels her vacation with her boyfriend, Wade. Unwilling to allow Barbara to continue neglecting her personal life, Alfred the Butler brings Wade to the clock tower and exposes her secret. Meanwhile, Helena goes to Arkham Asylum and interrogates a villain named Clayface, who possesses the same abilities as Chris, and learns a shocking fact about her past. She must race to prevent Chris from killing New Gotham's most powerful people at a charity fashion show.

Having discovered that Helena is Huntress, Harley Quinn uses a machine that gives her metahuman hypnotic powers. She then hypnotizes Helena and persuades her to knock Barbara unconscious and steal an access disk. Barbara recovers and uses her subneural device to enable her to stand and fight Helena, eventually neutralizing the trance. But Harley uses the disk to gain complete control of the clock tower and, after killing Wade, she broadcasts the hypnotic signal to every television set in New Gotham. Working together with Alfred, Dinah, and Reese, Helena must infiltrate the clock tower and battle Harley and her cohorts before they gain control of the entire city.